Expanding on a previous answer, this removes all new lines and saves the result to a new file (thanks to @tripleee):
tr -d '\n' < yourfile.txt > yourfile2.txt
Which is better than a "useless cat" (see comments):
cat file.txt | tr -d '\n' > file2.txt
Also useful for getting rid of new lines at the end of the file, e.g. created by using echo blah > file.txt
.