This works perfectly for adding rows and controls in a TableLayoutPanel.
Define a blank Tablelayoutpanel with 3 columns in the design page
Dim TableLayoutPanel3 As New TableLayoutPanel()
TableLayoutPanel3.Name = "TableLayoutPanel3"
TableLayoutPanel3.Location = New System.Drawing.Point(32, 287)
TableLayoutPanel3.AutoSize = True
TableLayoutPanel3.Size = New System.Drawing.Size(620, 20)
TableLayoutPanel3.ColumnCount = 3
TableLayoutPanel3.CellBorderStyle = TableLayoutPanelCellBorderStyle.Single
TableLayoutPanel3.BackColor = System.Drawing.Color.Transparent
TableLayoutPanel3.ColumnStyles.Add(New ColumnStyle(SizeType.Percent, 26.34146!))
TableLayoutPanel3.ColumnStyles.Add(New ColumnStyle(SizeType.Percent, 73.65854!))
TableLayoutPanel3.ColumnStyles.Add(New ColumnStyle(SizeType.Absolute, 85.0!))
Controls.Add(TableLayoutPanel3)
Create a button btnAddRow to add rows on each click
Private Sub btnAddRow_Click(sender As System.Object, e As System.EventArgs) Handles btnAddRow.Click
TableLayoutPanel3.GrowStyle = TableLayoutPanelGrowStyle.AddRows
TableLayoutPanel3.RowStyles.Add(New RowStyle(SizeType.Absolute, 20))
TableLayoutPanel3.SuspendLayout()
TableLayoutPanel3.RowCount += 1
Dim tb1 As New TextBox()
Dim tb2 As New TextBox()
Dim tb3 As New TextBox()
TableLayoutPanel3.Controls.Add(tb1 , 0, TableLayoutPanel3.RowCount - 1)
TableLayoutPanel3.Controls.Add(tb2, 1, TableLayoutPanel3.RowCount - 1)
TableLayoutPanel3.Controls.Add(tb3, 2, TableLayoutPanel3.RowCount - 1)
TableLayoutPanel3.ResumeLayout()
tb1.Focus()
End Sub