GameBoard is control of type DataGridView; The image should be type of PNG with transparent alpha channel background;
Image test = Properties.Resources.checker_black;
PictureBox b = new PictureBox();
b.Parent = GameBoard;
b.Image = test;
b.Width = test.Width*2;
b.Height = test.Height*2;
b.Location = new Point(0, 90);
b.BackColor = Color.Transparent;
b.BringToFront();