These are the two methods I use for speeding up my code:
For CPU bound applications:
For I/O bound applications:
N.B.
If you don't have a profiler, use the poor man's profiler. Hit pause while debugging your application. Most developer suites will break into assembly with commented line numbers. You're statistically likely to land in a region that is eating most of your CPU cycles.
For CPU, the reason for profiling in DEBUG mode is because if your tried profiling in RELEASE mode, the compiler is going to reduce math, vectorize loops, and inline functions which tends to glob your code into an un-mappable mess when it's assembled. An un-mappable mess means your profiler will not be able to clearly identify what is taking so long because the assembly may not correspond to the source code under optimization. If you need the performance (e.g. timing sensitive) of RELEASE mode, disable debugger features as needed to keep a usable performance.
For I/O-bound, the profiler can still identify I/O operations in RELEASE mode because I/O operations are either externally linked to a shared library (most of the time) or in the worst case, will result in a sys-call interrupt vector (which is also easily identifiable by the profiler).