This one worked for me:
>> print(df)
TotalVolume Symbol
2016-04-15 09:00:00 108400 2802.T
2016-04-15 09:05:00 50300 2802.T
>> print(df.set_index(pd.to_datetime(df.index.values) - datetime(2016, 4, 15)))
TotalVolume Symbol
09:00:00 108400 2802.T
09:05:00 50300 2802.T