[c#] Is there a way to check if a file is in use?

You can suffer from a thread race condition on this which there are documented examples of this being used as a security vulnerability. If you check that the file is available, but then try and use it you could throw at that point, which a malicious user could use to force and exploit in your code.

Your best bet is a try catch / finally which tries to get the file handle.

try
{
   using (Stream stream = new FileStream("MyFilename.txt", FileMode.Open))
   {
        // File/Stream manipulating code here
   }
} catch {
  //check here why it failed and ask user to retry if the file is in use.
}

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