[python-3.x] Python error message io.UnsupportedOperation: not readable

Use a+ to open a file for reading, writing as well as create it if it doesn't exist.

a+ Opens a file for both appending and reading. The file pointer is at the end of the file if the file exists. The file opens in the append mode. If the file does not exist, it creates a new file for reading and writing. -Python file modes

with open('"File.txt', 'a+') as file:
    print(file.readlines())
    file.write("test")

Note: opening file in a with block makes sure that the file is properly closed at the block's end, even if an exception is raised on the way. It's equivalent to try-finally, but much shorter.