if you are using mysqli and have two db_connection file. like first one is
define('HOST','localhost');
define('USER','user');
define('PASS','passs');
define('**DB1**','database_name1');
$connMitra = new mysqli(HOST, USER, PASS, **DB1**);
second one is
define('HOST','localhost');
define('USER','user');
define('PASS','passs');
define(**'DB2**','database_name1');
$connMitra = new mysqli(HOST, USER, PASS, **DB2**);
SO just change the name of parameter pass in mysqli like DB1 and DB2. if you pass same parameter in mysqli suppose DB1 in both file then second database will no connect any more. So remember when you use two or more connection pass different parameter name in mysqli function