Yep, you can simply point your src tag to a .m3u playlist file. A .m3u file is easy to construct -
#hosted mp3's need absolute paths but file system links can use relative paths
http://servername.com/path/to/mp3.mp3
http://servername.com/path/to/anothermp3.mp3
/path/to/local-mp3.mp3
-----UPDATE-----
Well, it turns out playlist m3u files are supported on the iPhone, but not on much else including Safari 5 which is kind of sad. I'm not sure about Android phones but I doubt they support it either since Chrome doesn't. Sorry for the misinformation.