The bug is probably somewhere else in your code, because it should work fine:
>>> 3 not in [2, 3, 4]
False
>>> 3 not in [4, 5, 6]
True
Or with tuples:
>>> (2, 3) not in [(2, 3), (5, 6), (9, 1)]
False
>>> (2, 3) not in [(2, 7), (7, 3), "hi"]
True