Try + (instancetype)appearance of UITableView:
Objective-C:
[[UITableView appearance] setSeparatorColor:[UIColor blackColor]]; // set your desired colour in place of "[UIColor blackColor]"
Swift 3.0:
UITableView.appearance().separatorColor = UIColor.black // set your desired colour in place of "UIColor.black"
Note: Change will reflect to all tables used in application.