Unfortunately I find none of the existing answers particularly satisfying.
Here is a straightforward and complete Python 3 solution, using the csv module.
import csv
with open('../resources/temp_in.csv', newline='') as f:
reader = csv.reader(f, skipinitialspace=True)
rows = list(reader)
print(rows)
Notice the skipinitialspace=True
argument. This is necessary since, unfortunately, OP's CSV contains whitespace after each comma.
Output:
[['This is the first line', 'Line1'], ['This is the second line', 'Line2'], ['This is the third line', 'Line3']]