Oh sure. Use a temp table. If you want a single, not-very-performant statement that "works" you can go with:
DELETE FROM MyTable WHERE NOT RowID IN
(SELECT
(SELECT TOP 1 RowID FROM MyTable mt2
WHERE mt2.Col1 = mt.Col1
AND mt2.Col2 = mt.Col2
AND mt2.Col3 = mt.Col3)
FROM MyTable mt)
Basically, for each row in the table, the sub-select finds the top RowID of all rows that are exactly like the row under consideration. So you end up with a list of RowIDs that represent the "original" non-duplicated rows.