dt.AsEnumerable()
.GroupBy(r => new { Col1 = r["Col1"], Col2 = r["Col2"] })
.Select(g =>
{
var row = dt.NewRow();
row["PK"] = g.Min(r => r.Field<int>("PK"));
row["Col1"] = g.Key.Col1;
row["Col2"] = g.Key.Col2;
return row;
})
.CopyToDataTable();