Generally, you can use the func(*tuple) syntax. You can even pass a part of the tuple, which seems like what you're trying to do here:
t = (2010, 10, 2, 11, 4, 0, 2, 41, 0)
dt = datetime.datetime(*t[0:7])
This is called unpacking a tuple, and can be used for other iterables (such as lists) too. Here's another example (from the Python tutorial):
>>> range(3, 6) # normal call with separate arguments
[3, 4, 5]
>>> args = [3, 6]
>>> range(*args) # call with arguments unpacked from a list
[3, 4, 5]