[python] How can I remove the last character of a string in python?

No need to use expensive regex, if barely needed then try- Use r'(/)(?=$)' pattern that is capture last / and replace with r'' i.e. blank character.

>>>re.sub(r'(/)(?=$)',r'','/home/ro/A_Python_Scripts/flask-auto/myDirectory/scarlett Johanson/1448543562.17.jpg/')
>>>'/home/ro/A_Python_Scripts/flask-auto/myDirectory/scarlett Johanson/1448543562.17.jpg'

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