[bash] Delete all lines beginning with a # from a file

you can directly edit your file with

sed -i '/^#/ d'

If you want also delete comment lines that start with some whitespace use

sed -i '/^\s*#/ d'

Usually, you want to keep the first line of your script, if it is a sha-bang, so sed should not delete lines starting with #!. also it should delete lines, that just contain only a hash but no text. put it all together:

sed -i '/^\s*\(#[^!].*\|#$\)/d'

To be conform with all sed variants you need to add a backup extension to the -i option:

sed -i.bak '/^\s*#/ d' $file
rm -Rf $file.bak