[c#] Why is access to the path denied?

When a user tries to connect to your Web site, IIS assigns the connection to the IUSER_ComputerName account, where ComputerName is the name of the server on which IIS is running. By default, the IUSER_ComputerName account is a member of the Guests group. This group has security restrictions. Try to grand access to IUSER_ComputerName to that folder

Here is very good described answer about IIS security

Hope this helps

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HTTP Error 401.2 - Unauthorized You are not authorized to view this page due to invalid authentication headers Why is access to the path denied?