f = open(filename,'r')
f.seek(-1,2) # go to the file end.
eof = f.tell() # get the end of file location
f.seek(0,0) # go back to file beginning
while(f.tell() != eof):
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You can use the file methods seek() and tell() to determine the position of the end of file. Once the position is found, seek back to the file beginning