If all images are of the same format:
import cv2
import glob
images = [cv2.imread(file) for file in glob.glob('path/to/files/*.jpg')]
For reading images of different formats:
import cv2
import glob
imdir = 'path/to/files/'
ext = ['png', 'jpg', 'gif'] # Add image formats here
files = []
[files.extend(glob.glob(imdir + '*.' + e)) for e in ext]
images = [cv2.imread(file) for file in files]