If the check box's ID "Delete" then for the "onclick" event of the submit button the javascript function can be as follows:
html:
<input type="checkbox" name="Delete" value="Delete" id="Delete"></td>
<input type="button" value="Delete" name="delBtn" id="delBtn" onclick="deleteData()">
script:
<script type="text/Javascript">
function deleteData() {
if(!document.getElementById('Delete').checked){
alert('Checkbox not checked');
return false;
}
</script>