Since you say you want to access by position once your data is read in, you should know about R's subsetting/ indexing functions.
The easiest is
df[row,column]
#example
df[1:5,] #rows 1:5, all columns
df[,5] #all rows, column 5.
Other methods are here. I personally use the dplyr package for intuitive data manipulation (not by position).