[asp.net] Problem in running .net framework 4.0 website on iis 7.0

Depending on the type of application, another thing to check is under the Advanced Settings for the Application Pool make sure "Enable 32-Bit Applications" is set to True.

I'd checked everything in this thread when I had this issue but all had already been setup correctly, I found this was the problem for me.

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Problem in running .net framework 4.0 website on iis 7.0