[python] Python Database connection Close

You might try turning off pooling, which is enabled by default. See this discussion for more information.

import pyodbc
pyodbc.pooling = False
conn = pyodbc.connect('DRIVER=MySQL ODBC 5.1 driver;SERVER=localhost;DATABASE=spt;UID=who;PWD=testest') 

csr = conn.cursor()  
csr.close()
del csr