If you are dealing with a lower level time object (I often just use integers), and don't want to write a custom filter for whatever reason, an approach I use is to pass the strftime function into the template as a variable, where it can be called where you need it.
For example:
import time
context={
'now':int(time.time()),
'strftime':time.strftime } # Note there are no brackets () after strftime
# This means we are passing in a function,
# not the result of a function.
self.response.write(jinja2.render_template('sometemplate.html', **context))
Which can then be used within sometemplate.html
:
<html>
<body>
<p>The time is {{ strftime('%H:%M%:%S',now) }}, and 5 seconds ago it was {{ strftime('%H:%M%:%S',now-5) }}.
</body>
</html>