Each application pool in IIs creates its own secure user folder with FULL read/write permission by default under c:\users. Open up your Users folder and see what application pool folders are there, right click, and check their rights for the application pool virtual account assigned. You should see your application pool account added already with read/write access assigned to its root and subfolders.
So that type of file storage access is automatically done and you should be able to write whatever you like there in the app pools user account folders without changing anything. That's why virtual user accounts for each application pool were created.