One thing to ensure is that nothing can reassign your variable. If it is not a Boolean in the end, relying on truthiness will lead to bugs. The beauty of conditional programming in dynamically typed languages :).
The following prints "no".
x = False
if x:
print 'yes'
else:
print 'no'
Now let's change x.
x = 'False'
Now the statement prints "yes", because the string is truthy.
if x:
print 'yes'
else:
print 'no'
This statement, however, correctly outputs "no".
if x == True:
print 'yes'
else:
print 'no'