One of the values you pass on to Ancestors
becomes None
at some point, it says, so check if otu
, tree
, tree[otu]
or tree[otu][0]
are None
in the beginning of the function instead of only checking tree[otu][0][0] == None
. But perhaps you should reconsider your path of action and the datatype in question to see if you could improve the structure somewhat.