[python] How to correct "TypeError: 'NoneType' object is not subscriptable" in recursive function?

One of the values you pass on to Ancestors becomes None at some point, it says, so check if otu, tree, tree[otu] or tree[otu][0] are None in the beginning of the function instead of only checking tree[otu][0][0] == None. But perhaps you should reconsider your path of action and the datatype in question to see if you could improve the structure somewhat.