There is now a an approach to do this, using Doctrine's Criteria.
A full example can be seen in How to use a findBy method with comparative criteria, but a brief answer follows.
use \Doctrine\Common\Collections\Criteria;
// Add a not equals parameter to your criteria
$criteria = new Criteria();
$criteria->where(Criteria::expr()->neq('prize', 200));
// Find all from the repository matching your criteria
$result = $entityRepository->matching($criteria);