Yes, just add multiple FileAppenders to your logger. For example:
<log4net>
<appender name="File1Appender" type="log4net.Appender.FileAppender">
<file value="log-file-1.txt" />
<appendToFile value="true" />
<layout type="log4net.Layout.PatternLayout">
<conversionPattern value="%date %message%newline" />
</layout>
</appender>
<appender name="File2Appender" type="log4net.Appender.FileAppender">
<file value="log-file-2.txt" />
<appendToFile value="true" />
<layout type="log4net.Layout.PatternLayout">
<conversionPattern value="%date %message%newline" />
</layout>
</appender>
<root>
<level value="DEBUG" />
<appender-ref ref="File1Appender" />
<appender-ref ref="File2Appender" />
</root>
</log4net>